铜(Ⅱ)配合物{[Cu(OMBA)2]2·(DMF)2}的合成、CT-DNA结合及细胞毒性

摘要/Abstract

摘要： 合成了配合物{[Cu(OMBA)₂]₂·(DMF)₂}(OMBA =邻甲基苯甲酸,DMF = N,N-二甲基甲酰胺),配合物结构经元素分析、IR和X射线单晶衍射表征,该配合物属于三斜晶系,P1空间群,晶胞参数为a=1.037 0(5) nm, b=1.053 6(4) nm, c=1.105 9(5) nm, α=62.737(6)°, β=73.355(7)°, γ=63.231(7)°, Z=1, D_c=1.416 g·cm^-3, F(000)=422,最终结构残差因子R₁=0.051 5, wR₂=0.140 4。采用紫外、荧光和黏度法研究了配合物和小牛胸腺DNA (CT-DNA)的相互作用方式。结果表明,配合物与DNA的结合常数K_b=939.61 L·mol^-1,K_sv=3.00×10³ L·mol^-1,猝灭速率常数K_q=3.00×10¹¹ L·mol^-1·s^-1。配合物静态猝灭CT-DNA的荧光,结合常数K_a=5.38×10³ L·mol^-1,结合位点n=1,配合物对胃癌细胞A549、宫颈癌细胞Hela和肝癌细胞HepG2有抗增殖作用。

关键词: 铜(Ⅱ), 配合物, 合成, DNA结合, 细胞毒性, 抗肿瘤活性, DNA复制

Abstract: In this paper, a copper(Ⅱ) complex {[Cu(OMBA)₂]₂·(DMF)₂} (OMBA=o-methylbenzoic acid, DMF = N,N-dimethylformamide) was synthesized. The structure of the complex was characterized using elemental analysis, infrared (IR) and X-ray single crystal diffraction. The complex belongs to triclinic, P1 space group, with a=1.037 0(5) nm, b=1.053 6(4) nm, c=1.105 9(5) nm, α=62.737(6)°, β=73.355(7)°, γ=63.231(7)°, Z=1, D_c=1.416 g·cm^-3, F(000)=422 unit cell parameters. The final R₁=0.051 5, wR₂=0.140 4. The interaction between CT-DNA (calf thymus DNA) and the complex was evaluated by UV-Vis, fluorescence spectroscopy, and viscometry. When the complex bound to CT-DNA, the binding constant K_b=939.61 L·mol^-1, K_sv=3.00×10³ L·mol^-1, and K_q=3.00×10¹¹ L·mol^-1·s^-1. The complex quenched the intrinsic fluorescence of CT-DNA via static quenching, K_a=5.38×10³ L·mol^-1, n=1. The complex has anti-proliferation effects on A549, Hela and HepG2.

Key words: copper(Ⅱ), complex, synthesis, DNA binding, cytotoxic, antitumor activity, DNA replication

中图分类号:

O614.1

曾振芳, 蔡杰慧, 黄秋萍, 韦友欢, 陈丽苗, 黄秋婵. 铜(Ⅱ)配合物{[Cu(OMBA)₂]₂·(DMF)₂}的合成、CT-DNA结合及细胞毒性[J]. 人工晶体学报, 2021, 50(12): 2300-2306.

ZENG Zhenfang, CAI Jiehui, HUANG Qiuping, WEI Youhuan, CHEN Limiao, HUANG Qiuchan. Synthesis, CT-DNA Binding, and Cytotoxic Activity of Copper(Ⅱ) Complex {[Cu(OMBA)₂]₂·(DMF)₂}[J]. JOURNAL OF SYNTHETIC CRYSTALS, 2021, 50(12): 2300-2306.

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铜(Ⅱ)配合物{[Cu(OMBA)₂]₂·(DMF)₂}的合成、CT-DNA结合及细胞毒性

Synthesis, CT-DNA Binding, and Cytotoxic Activity of Copper(Ⅱ) Complex {[Cu(OMBA)₂]₂·(DMF)₂}

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