对氯苯甲酸构筑的铜(Ⅱ)配合物的合成、HSA结合及细胞毒性

人工晶体学报 ›› 2022, Vol. 51 ›› Issue (1): 126-131.

对氯苯甲酸构筑的铜(Ⅱ)配合物的合成、HSA结合及细胞毒性

曾振芳, 袁芳, 黄秋萍, 庞华钰, 杨红兰, 黄秋婵

广西民族师范学院化学与生物工程学院,崇左 532200

收稿日期:2021-11-02 出版日期:2022-01-15 发布日期:2022-02-09
通讯作者: 黄秋婵,教授。E-mail:huangqiuchan2021@163.com
作者简介:曾振芳(1984—),女,广西壮族自治区人,副教授。E-mail:251292662@qq.com
基金资助:
广西自然科学基金(2020JJB130043);广西教育厅高校科研项目(2017KY0846);广西民族师范学院科研项目(2020JS002,2021YB036,2021YB038,2019YB018,2020GP008)

Synthesis, HSA Binding, and Cytotoxic Activity of Copper(Ⅱ) Complex Constructed by P-Chlorobenzoic Acid

ZENG Zhenfang, YUAN Fang, HUANG Qiuping, PANG Huayu, YANG Honglan, HUANG Qiuchan

School of Chemical and Biological Engineering, Guangxi Normal University for Nationalities, Chongzuo 532200, China

Received:2021-11-02 Online:2022-01-15 Published:2022-02-09

摘要/Abstract

摘要： 本文合成了配合物[Cu(pcba)₂·(phen)(H₂O)] (pcba =对氯苯甲酸,phen = 1,10-邻菲罗啉),该配合物属于三斜晶系,P1空间群,晶胞参数为a=0.790 98(2) nm,b=1.072 40(4) nm,c=1.487 19(6) nm,α=100.613(3)°,β=95.239(3)°,γ=108.334(3)°,Z=2,D_c=1.638 g·cm^-3,F(000)=582,最终结构残差因子R₁=0.035 9,wR₂=0.089 1。采用紫外及荧光研究了配合物和人血清蛋白(HSA)的相互作用方式。结果表明,配合物静态猝灭HSA荧光,可求得配合物与HSA的猝灭常数K_sv=2.35×10⁵ L·mol^-1,猝灭速率常数K_q=2.35×10¹³ L·mol^-1·s^-1,结合常数为K_a=2.14×10¹³ L·mol^-1,结合位点n=2.37。同时,研究了配合物对胃癌细胞A549、宫颈癌细胞Hela和肝癌细胞HepG2的抗增殖能力。

关键词: 配合物, 铜金属配合物, 人血清蛋白, 对氯苯甲酸, 铜(Ⅱ), HSA结合, 细胞毒性

Abstract: In this paper, a copper(Ⅱ) complex [Cu(pcba)₂·(phen)(H₂O)] (pcba=p-chlorbenzoic acid, phen=1,10-phenanthroline) was synthesized. The structure of the complex belongs to triclinic, P1 space group, with a=0.790 98(2) nm,b=1.072 40(4) nm, c=1.487 19(6) nm, α=100.613(3)°, β=95.239(3)°, γ=108.334(3)°, Z=2, D_c=1.638 g·cm^-3, F(000)=582 unit cell parameters. The final R₁=0.035 9, wR₂=0.089 1. The interaction between HSA (human serum albumin) and the complex was evaluated by UV-Vis and fluorescence spectroscopy. The complex quenched the intrinsic fluorescence of HSA via static quenching with K_sv=2.35×10⁵ L·mol^-1, the quenching rate constant K_q=2.35×10¹³ L·mol^-1·s^-1, the binding constant K_a=2.14×10¹³ L·mol^-1, the binding site n=2.37. Furthermore, the proliferation inhibition effect of the complex on the cells A549, Hela and HepG2 was studied.

Key words: complex, copper complex, human serum albumi, P-chlorobenzoic acid, copper(Ⅱ), HSA binding, cytotoxic activity

中图分类号:

曾振芳, 袁芳, 黄秋萍, 庞华钰, 杨红兰, 黄秋婵. 对氯苯甲酸构筑的铜(Ⅱ)配合物的合成、HSA结合及细胞毒性[J]. 人工晶体学报, 2022, 51(1): 126-131.

ZENG Zhenfang, YUAN Fang, HUANG Qiuping, PANG Huayu, YANG Honglan, HUANG Qiuchan. Synthesis, HSA Binding, and Cytotoxic Activity of Copper(Ⅱ) Complex Constructed by P-Chlorobenzoic Acid[J]. JOURNAL OF SYNTHETIC CRYSTALS, 2022, 51(1): 126-131.

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