Synthesis of Ag-Chitosan by Green Route and Its Isotherms, Kinetic and Antimicrobial Activity

Abstract

Abstract: A green route, namely adsorption methods, has been developed to synthesize silver-loaded chitosan(Ag-chitosan). The microstructure and thermal stability were studied by X-ray diffraction (XRD), field emission scanning electric mirror (FESEM) and thermal weight analysis. The adsorption model of chitosan on silver was also studied. The antibacterial effect of Ag-chitosan was evaluated by plate diffusion board method. The results show that the introduction of silver ions in the polysaccharide structure affects its original crystal structure and improves its thermal stability. The adsorption isotherm of chitosan to silver ions is consistent with the Langmuir and Dubinin-Radushkevich equations, indicating that the adsorption process of silver ions in chitosan complies with single layer adsorption and chemical adsorption, and the maximum adsorption of chitosan to silver ions is 478.09 mg/g, silver content is 32.34%, which is consistent with the Dubinin-Radushkevich calculation. Therefore, chitosan with 32.34% silver content was obtained and tested against Escherichia coli and Staphylococcus aureus, which is considered one of the most widespread wound burn infectious bacteria. The antibacterial test results indicate that Ag-chitosan has favourable antibacterial property and MIC≤80 μg/mL against Escherichia coli and Staphylococcus aureus.

Key words: silver-loaded chitosan, green route, adsorption, isotherm, antibacterial property, antibacterial material

CLC Number:

O636.1

WANG Zuhua, LIU Ping, YANG Ruixian, CHEN Huajun. Synthesis of Ag-Chitosan by Green Route and Its Isotherms, Kinetic and Antimicrobial Activity[J]. JOURNAL OF SYNTHETIC CRYSTALS, 2021, 50(12): 2307-2315.

References

[1] 卢凤琦,曹宗顺,庄昭霞,等.壳聚糖膜的降解与生物相容性研究[J].生物医学工程学杂志,1998,15(2):183-185.
LU F Q, CAO Z S, ZHUANG Z X, et al. Biodegradation and biocompatibility of a chitosan film[J]. Journal of Biomedical Engineering, 1998, 15(2): 183-185(in Chinese).
[2] KONG M, CHEN X G, XING K, et al. Antimicrobial properties of chitosan and mode of action: a state of the art review[J]. International Journal of Food Microbiology, 2010, 144(1): 51-63.
[3] RINAUDO M. Chitin and chitosan: properties and applications[J]. Progress in Polymer Science, 2006, 31(7): 603-632.
[4] 周志敏,裴继诚,刘海棠,等.漆酶/TEMPO体系氧化棉纤维接枝壳聚糖制备抗菌纤维[J].功能材料,2018,49(6):6140-6145.
ZHOU Z M, PEI J C, LIU H T, et al. Antibacterial modification of cotton fabric with chitosan via laccase/TEMPO mediated grafting[J]. Journal of Functional Materials, 2018, 49(6): 6140-6145(in Chinese).
[5] 裴继诚,殷允北,胡稳,等.漆酶-TEMPO体系选择性氧化壳聚糖C-6伯羟基提高其抗氧化性能的研究[J].功能材料,2016,47(6):6010-6015.
PEI J C, YIN Y B, HU W, et al. Study on the selective oxidation of the C-6-hydroxyl of chitosan by laccase-TEMPO system to improve antioxidant capability of the product[J]. Journal of Functional Materials, 2016, 47(6): 6010-6015(in Chinese).
[6] 甄小琴,郝东昭,裴继诚,等.漆酶/TEMPO修饰壳寡糖及其制备产物对黑色素抑制效果的研究[J].功能材料,2019,50(3):3008-3013.
ZHEN X Q, HAO D Z, PEI J C, et al. The study of chitooligomer modified by laccase/TEMPO system and its inhibitory effect on melanin[J]. Journal of Functional Materials, 2019, 50(3): 3008-3013(in Chinese).
[7] JAISWAL N, SINHA D J, SINGH U P, et al. Evaluation of antibacterial efficacy of chitosan, chlorhexidine, propolis and sodium hypochlorite on enterococcus faecalis biofilm: an in vitro study[J]. Journal of Clinical and Experimental Dentistry, 2017, 9(9): e1066-e1074.
[8] MITRA D, LI M, KANG E T, et al. Transparent copper-loaded chitosan/silica antibacterial coatings with long-term efficacy[J]. ACS Applied Materials & Interfaces, 2017, 9(35): 29515-29525.
[9] RABEA E I, BADAWY M E I, STEURBAUT W, et al. In vitro assessment of N-(benzyl)chitosan derivatives against some plant pathogenic bacteria and fungi[J]. European Polymer Journal, 2009, 45(1): 237-245.
[10] CHO Y I, NO H K, MEYERS S P. Physicochemical characteristics and functional properties of various commercial chitin and chitosan products[J]. Journal of Agricultural and Food Chemistry, 1998, 46(9): 3839-3843.
[11] SINGH D K, RAY A R. Biomedical applications of chitin, chitosan, and their derivatives[J]. Journal of Macromolecular Science, Part C, 2000, 40(1): 69-83.
[12] PINTO R J B, FERNANDES S C M, FREIRE C S R, et al. Antibacterial activity of optically transparent nanocomposite films based on chitosan or its derivatives and silver nanoparticles[J]. Carbohydrate Research, 2012, 348: 77-83.
[13] SINGH M. Elucidation of biogenic silver nanoparticles susceptibility towards Escherichia coli: an investigation on the antimicrobial mechanism[J]. IET Nanobiotechnology, 2016, 10(5): 276-280.
[14] HOLT K B, BARD A J. Interaction of silver(I) ions with the respiratory chain of Escherichia coli: an electrochemical and scanning electrochemical microscopy study of the antimicrobial mechanism of micromolar Ag⁺[J]. Biochemistry, 2005, 44(39): 13214-13223.
[15] 蔡波,陈宇媚,冷军程,等.改性壳聚糖基纳米银复合材料的制备与抗菌性能[J].材料科学,2018,8(4):265-272.
CAI B, CHEN Y M, LENG J C, et al. Synthesis and anti/bacteriostasis of nano-silver composites based modified chitosan[J].Material Sciences, 2018, 8(4): 265-272(in Chinese).
[16] 安静,郭桂真,罗青枝,等.载银壳聚糖接枝香草醛胶乳的制备及抗菌性[J].河北科技大学学报,2016,37(4):357-363.
AN J, GUO G Z, LUO Q Z, et al. Preparation and the antibacterial activity of silver-loaded chitosan-grafted-vanillin sol[J]. Journal of Hebei University of Science and Technology, 2016, 37(4): 357-363(in Chinese).
[17] LI Q, LU F, ZHOU G, et al. Silver inlaid with gold nanoparticle/chitosan wound dressing enhances antibacterial activity and porosity, and promotes wound healing[J]. Biomacromolecules, 2017, 18(11): 3766-3775.
[18] REGIEL-FUTYRA A, KUS-LIŚKIEWICZ M, SEBASTIAN V, et al. Development of noncytotoxic silver-chitosan nanocomposites for efficient control of biofilm forming microbes[J]. RSC Advances, 2017, 7(83): 52398-52413.
[19] 胡芳,葛红岩,张策,等.载银壳聚糖涂布液的制备及涂布纸抑菌性能的研究[J].中华纸业,2015,36(12):12-16.
HU F, GE H Y, ZHANG C, et al. Preparation and properties of silver-carried chitosan coated antibacterial paper[J]. China Pulp & Paper Industry, 2015, 36(12): 12-16(in Chinese).
[20] WU Y, ZHENG Y L, YANG W L, et al. Synthesis and characterization of a novel amphiphilic chitosan-polylactide graft copolymer[J]. Carbohydrate Polymers, 2005, 59(2): 165-171.
[21] 蒋卫娟,关静,黄震.载银壳聚糖微球的制备及其抗菌性研究[J].包装工程,2012,33(21):15-19+40.
JIANG W J, GUAN J, HUANG Z. Preparation and antibacterial performance evaluation of silver loaded chitosan microsphere[J]. Packaging Engineering, 2012, 33(21): 15-19+40(in Chinese).
[22] 牛梅,戴晋明,侯文生,等.载银壳聚糖复合物的结构及其抗菌性能研究[J].材料导报,2011,25(10):15-18+26.
NIU M, DAI J M, HOU W S, et al. Study on the structure and antibacterial activity of Ag-loading chitosan composites[J]. Materials Review, 2011, 25(10): 15-18+26(in Chinese).
[23] HANG A T, TAE B, PARK J S. Non-woven mats of poly(vinyl alcohol)/chitosan blends containing silver nanoparticles: fabrication and characterization[J]. Carbohydrate Polymers, 2010, 82(2): 472-479.
[24] IGNATOVA M, STARBOVA K, MARKOVA N, et al. Electrospun nano-fibre mats with antibacterial properties from quaternised chitosan and poly(vinyl alcohol)[J]. Carbohydrate Research, 2006, 341(12): 2098-2107.
[25] AN J, ZHANG H, ZHANG J T, et al. Preparation and antibacterial activity of electrospun chitosan/poly(ethylene oxide) membranes containing silver nanoparticles[J]. Colloid and Polymer Science, 2009, 287(12): 1425-1434.