Affecting Analysis of Multi-Factors on Soybean Urease-Induced Calcium Carbonate Precipitation

Abstract

Abstract: The effects of multiple influencing factors on soybean urease-induced calcium carbonate precipitation (SICP) were explored to select the main influencing factors and provided their optimal ranges. Firstly, the influence of urease concentration and temperature on urease activity were analyzed. After that, based on orthogonal test design, 25 test conditions of SICP aqueous solution tests were conducted, and the variation law of Ca²⁺ utilization ratio was studied through the combination of different factors. Also the morphology of CaCO₃ was observed with SEM in diverse test conditions. The results show that low temperature delivers benefits to urease preservation and its activity exertion, urease activity can be preserved over 21 d with 5 ℃. At the same temperature, the greater the urease concentration is, the higher the initial activity of urease boasts and the shorter the time required for complete inactivation of urease. The pH value and the volume ratio of urease to cementation solution are the main factors affecting the Ca²⁺ utilization ratio. In order to achieve a higher Ca²⁺ utilization ratio, the optimum volume ratio of urease to cementation solution could be 1 while the optimal concentration ratio of CaCl₂ to urea is 1.5, and the best Ca²⁺ concentration is 1 mol/L. More hexahedral CaCO₃ is produced, when the urease concentration is low, and the shape of precipitated CaCO₃ turns into spherical as the urease concentration increases. Aspartic acid which is rich in soybeans, is an significant factor in controlling the form of CaCO₃.

Key words: soybean urease-induced calcium carbonate precipitation, Ca²⁺ utilization ratio, spherical CaCO₃, aspartic acid, multi-factors, orthogonal test

CLC Number:

TU41

YUAN Hua, LIU Kang, YUAN Yaonan, FENG Jiaxing. Affecting Analysis of Multi-Factors on Soybean Urease-Induced Calcium Carbonate Precipitation[J]. JOURNAL OF SYNTHETIC CRYSTALS, 2021, 50(2): 375-380.

References

[1] RAN D, KAWASAKI S. Effective use of plant-derived urease in the field of geoenvironmental/geotechnical engineering[J]. Journal of Civil & Environmental Engineering, 2016, 6(1): 1-13.
[2] HAMDAN N, KAVAZANJIAN E Jr. Enzyme-induced carbonate mineral precipitation for fugitive dust control[J]. Géotechnique, 2016, 66(7): 546-555.
[3] 吴敏,高玉峰,何稼,等.大豆脲酶诱导碳酸钙沉积与黄原胶联合防风固沙室内试验研究[J].岩土工程学报,2020,42(10):1914-1921.
WU M, GAO Y F, HE J, et al. Laboratory study on use of soybean urease-induced calcium carbonate precipitation with xanthan gum for stabilization of desert sand against wind erosion[J]. Chinese Journal of Geotechnical Engineering, 2020, 42(10): 1914-1921(in Chinese).
[4] DILRUKSHI R A N, NAKASHIMA K, KAWASAKI S. Soil improvement using plant-derived urease-induced calcium carbonate precipitation[J]. Soils and Foundations, 2018, 58(4): 894-910.
[5] CUI M J, LAI H J, HOANG T, et al. One-phase-low-pH enzyme induced carbonate precipitation (EICP) method for soil improvement[J]. Acta Geotechnica, 2020: 1-9.
[6] 任冠洲,原华,刘康,等.大豆脲酶诱导碳酸钙沉积技术反应液配方试验[J].中国科技论文,2020,15(9):1085-1089+1098.
REN G Z, YUAN H, LIU K, et al. Experimental study on reaction solution formulation of soybean urease induced calcium carbonate deposition technology[J]. ChinaSciencepaper, 2020, 15(9): 1085-1089+1098(in Chinese).
[7] 吴林玉,缪林昌,孙潇昊,等.植物源脲酶诱导碳酸钙固化砂土试验研究[J].岩土工程学报,2020,42(4):714-720.
WU L Y, MIAO L C, SUN X H, et al. Experimental study on sand solidification using plant-derived urease-induced calcium carbonate precipitation[J]. Chinese Journal of Geotechnical Engineering, 2020, 42(4): 714-720(in Chinese).
[8] WHIFFIN V S. Microbial CaCO₃ precipitation for the production of biocement[EB/OL].
[9] 沈吉云.微生物成因土工材料实验及应用研究[D].北京:清华大学,2009.
SHEN J Y. Experiments and applications of bio-geomaterials[D]. Beijing: Tsinghua University, 2009(in Chinese).
[10] TERZIS D, BERNIER-LATMANI R, LALOUI L. Fabric characteristics and mechanical response of bio-improved sand to various treatment conditions[J]. Géotechnique Letters, 2016, 6(1): 50-57.
[11] CARMONA J P S F, VENDA OLIVEIRA P J, LEMOS L J L, et al. Improvement of a sandy soil by enzymatic calcium carbonate precipitation[J]. Proceedings of the Institution of Civil Engineers-Geotechnical Engineering, 2018, 171(1): 3-15.
[12] 陈传宝,张群,方亮.天冬氨酸对碳酸钙形貌和晶型的控制[J].安庆师范学院学报(自然科学版),2008,14(2):45-47.
CHEN C B, ZHANG Q, FANG L.Control of DL-aspartic acid on morphology and structure of calcium carbonate[J]. Journal of Anqing Teachers College (Natural Science Edition), 2008, 14(2): 45-47(in Chinese).
[13] SUGIYAMA S, TAKASAKI M, OAKI Y, et al. Strained calcite crystals from amorphous calcium carbonate containing an organic molecule[J]. CrystEngComm, 2020, 22(42): 7054-7058.
[14] SONDI I, ŠKAPIN S D, SALOPEK-SONDI B. Biomimetic precipitation of nanostructured colloidal calcite particles by enzyme-catalyzed reaction in the presence of magnesium ions[J]. Crystal Growth & Design, 2008, 8(2): 435-441.
[15] 胡名益,黄梦萍,金胜明,等.L-天冬氨酸诱导合成球霰石工艺研究[J].矿冶工程,2019,39(2):93-96+101.
HU M Y, HUANG M P, JIN S M, et al. Technical process for synthesis ofvaterite calcium carbonate by using L-aspartic acid[J]. Mining and Metallurgical Engineering, 2019, 39(2): 93-96+101(in Chinese).
[16] 王俊,王艳玲,施伟光,等.可溶性添加剂对碳酸钙晶型及形貌控制的研究进展[J].硅酸盐通报,2015,34(12):3523-3528.
WANG J, WANG Y L, SHI W G, et al.Progress of the effect of water-soluble additives on the polymorph and morphology of calcium carbonate[J]. Bulletin of the Chinese Ceramic Society, 2015, 34(12): 3523-3528(in Chinese).
[17] 徐焕焕,华睿清,吴刚.球形方解石的合成和表征[J].人工晶体学报,2019,48(11):2141-2145.
XU H H, HUA R Q, WU G. Synthesis and characterization of spherical-like calcite[J]. Journal of Synthetic Crystals, 2019, 48(11): 2141-2145(in Chinese).