Preparation of Resveratrol Nano-Delivery System Based on Mesoporous Silica

Abstract

Abstract: The purpose of this experiment was to construct a mesoporous silicon-encapsulated resveratrol nano-delivery system. The nanosystem before and after loading resveratrol was characterized and analyzed by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, N₂ adsorption-desorption, differential thermal-thermogravimetric analysis. Its light and heat stability properties and release laws in different pH buffers in vitro were explored. The results show that the mesoporous silica-encapsulated resveratrol nano-delivery system has a cross-linked network structure. The pore size, specific surface area and pore volume are 10.16 nm, 245.73 m²/g and 0.79 cm³/g, respectively, and the loading capacity could reach 39.44%. The framework structure is not destroyed after loading, and the optical and thermal stability of resveratrol is effectively improved. Meanwhile, the release rate of resveratrol after nanosystem loading is more than 80% within 2 h in two different pH buffers. The mesoporous silica-encapsulated resveratrol nanosystem has better performance and provides reference significance for the further development and utilization of resveratrol.

Key words: resveratrol, mesoporous silica, nano-delivery system, drug loading capacity, release rate, stability

CLC Number:

TQ460.4

ZHANG Wenjun, LI Xiang, ZHANG Guofeng, WANG Qing, LYU Chunyan, LIU Yang. Preparation of Resveratrol Nano-Delivery System Based on Mesoporous Silica[J]. JOURNAL OF SYNTHETIC CRYSTALS, 2021, 50(12): 2323-2331.

References

[1] HOU C Y, TAIN Y L, YU H R, et al. The effects of resveratrol in the treatment of metabolic syndrome[J]. International Journal of Molecular Sciences, 2019, 20(3): 535.
[2] RATZ-ŁYKO A, ARCT J. Resveratrol as an active ingredient for cosmetic and dermatological applications: a review[J]. Journal of Cosmetic and Laser Therapy, 2019, 21(2): 84-90.
[3] MALAGUARNERA. Influence of resveratrol on the immune response[J]. Nutrients, 2019, 11(5): 946.
[4] SUI X, ZHANG C, JIANG Y, et al. Resveratrol activates DNA damage response through inhibition of polo-like kinase 1 (PLK1) in natural killer/T cell lymphoma[J]. Annals of Translational Medicine, 2020, 8(11): 688.
[5] ZHANG W, JIANG H T, CHEN Y J, et al. Resveratrol chemosensitizes adriamycin-resistant breast cancer cells by modulating miR-122-5p[J]. Journal of Cellular Biochemistry, 2019, 120(9): 16283-16292.
[6] RAUF A, IMRAN M, BUTT M S, et al. Resveratrol as an anti-cancer agent: a review[J]. Critical Reviews in Food Science and Nutrition, 2018, 58(9): 1428-1447.
[7] XU G R, CHEN J Z, WANG G R, et al. Resveratrol inhibits the tumorigenesis of follicular thyroid cancer via ST6GAL2-regulated activation of the hippo signaling pathway[J]. Molecular Therapy-Oncolytics, 2020, 16: 124-133.
[8] KO J H, SETHI G, UM J Y, et al. The role of resveratrol in cancer therapy[J]. International Journal of Molecular Sciences, 2017, 18(12): 2589.
[9] CHIMENTO A, DE AMICIS F, SIRIANNI R, et al. Progress to improve oral bioavailability and beneficial effects of resveratrol[J]. International Journal of Molecular Sciences, 2019, 20(6): 1381.
[10] 于琛琛,张纯刚,尹丽,等.白藜芦醇/羟丙基-β-环糊精/壳聚糖缓释微球的体外释放度及其在大鼠体内药动学的考察[J].中国药房,2019,30(19):2603-2607.
YU C C, ZHANG C G, YIN L, et al. Investigation in vitro release rate and in vivo pharmacokinetics of resveratrol/hydroxypropyl-β-cyclodextrin/chitosan sustained-release pellets in rats[J]. China Pharmacy, 2019, 30(19): 2603-2607(in Chinese).
[11] ZHANG L, ZHU K, ZENG H, et al. Resveratrol solid lipid nanoparticles to trigger credible inhibition of doxorubicin cardiotoxicity[J]. International Journal of Nanomedicine, 2019, 14: 6061-6071.
[12] NADIMI A E, EBRAHIMIPOUR S Y, AFSHAR E G, et al. Nano-scale drug delivery systems for antiarrhythmic agents[J]. European Journal of Medicinal Chemistry, 2018, 157: 1153-1163.
[13] ASHFAQ U A, RIAZ M, YASMEEN E, et al. Recent advances in nanoparticle-based targeted drug-delivery systems against cancer and role of tumor microenvironment[J]. Critical Reviews in Therapeutic Drug Carrier Systems, 2017, 34(4): 317-353.
[14] PATRA J K, DAS G, FRACETO L F, et al. Nano based drug delivery systems: recent developments and future prospects[J]. Journal of Nanobiotechnology, 2018, 16(1): 1-33.
[15] SUN M C, XU X L, LOU X F, et al. Recent progress and future directions: the nano-drug delivery system for the treatment of vitiligo[J]. International Journal of Nanomedicine, 2020, 15: 3267-3279.
[16] SU S, M KANG P. Recent advances in nanocarrier-assisted therapeutics delivery systems[J]. Pharmaceutics, 2020, 12(9). DOI:10.3390/pharmaceutics12090837.
[17] 张文君,吴梦婷,张国锋,等.白藜芦醇纳米传递系统的研究进展[J/OL].食品科学:1-11 [2021-02-06]. http://kns.cnki.net/kcms/detail/11.2206.TS.20201212.0811.004.html.
ZHANG W J, WU M T, ZHANG G F, et al. Research progress of resveratrol nano-delivery system [J / OL]. Food Science: 1-11 [2021-10-05] http://kns.cnki.net/kcms/detail/11.2206.TS.20201212.0811.004.html(in Chinese).
[18] BERNARDOS A, PIACENZA E, SANCENÓN F, et al. Mesoporous silica-based materials with bactericidal properties[J]. Small, 2019, 15(24): 1900669.
[19] JAFARI S, DERAKHSHANKHAH H, ALAEI L, et al. Mesoporous silica nanoparticles for therapeutic/diagnostic applications[J]. Biomedicine & Pharmacotherapy, 2019, 109: 1100-1111.
[20] 马博乐,陈雨晴,祝星宇,等.介孔二氧化硅纳米粒的功能化修饰及其在药物研究中的应用[J].中国药房,2018,29(15):2156-2160.
MA B L, CHEN Y Q, ZHU X Y, et al. Functional modification of mesoporous silica nanoparticles and its application in drug research[J]. China Pharmacy, 2018, 29(15): 2156-2160(in Chinese).
[21] WANG Y, ZHAO Q F, HAN N, et al. Mesoporous silica nanoparticles in drug delivery and biomedical applications[J]. Nanomedicine: Nanotechnology, Biology and Medicine, 2015, 11(2): 313-327.
[22] SUMMERLIN N, QU Z, PUJARA N, et al. Colloidal mesoporous silica nanoparticles enhance the biological activity of resveratrol[J]. Colloids and Surfaces B: Biointerfaces, 2016, 144: 1-7.
[23] DÍAZ-RUIZ R, MARTÍNEZ-REY L, LACA A, et al. Enhancing trans-Resveratrol loading capacity by forcing W1/O/W2 emulsions up to its colloidal stability limit[J]. Colloids and Surfaces B: Biointerfaces, 2020, 193: 111130.
[24] COTEA V V, LUCHIAN C E, BILBA N, et al. Mesoporous silica SBA-15, a new adsorbent for bioactive polyphenols from red wine[J]. Analytica Chimica Acta, 2012, 732: 180-185.
[25] SHEN Y, CAO B, SNYDER N R, et al. ROS responsive resveratrol delivery from LDLR peptide conjugated PLA-coated mesoporous silica nanoparticles across the blood-brain barrier[J]. Journal of Nanobiotechnology, 2018, 16(1): 13.
[26] 董玉洁,蒋沅岐,陈金鹏,等.中药缓控释制剂的研究进展[J].中草药,2021,52(8):2465-2472.
DONG Y J, JIANG Y Q, CHEN J P, et al. Research progress on sustained and controlled-release preparation of traditional Chinese medicine[J]. Chinese Traditional and Herbal Drugs, 2021, 52(8): 2465-2472(in Chinese).
[27] 李胜男,王计瑞,张继琼,等.浸渍离心法制备不同难溶性药物介孔二氧化硅纳米粒载药规律及机制研究[J].中草药,2017,48(13):2638-2644.
LI S N, WANG J R, ZHANG J Q, et al. Law and mechanism of incorporating different insoluble drugs into mesoporous silica nanoparticles by dipping centrifugation method[J]. Chinese Traditional and Herbal Drugs, 2017, 48(13): 2638-2644(in Chinese).
[28] 陈杰,姜海峰,马明硕,等.介孔二氧化硅花球的制备及药物缓释性能研究[J].化工技术与开发,2019,48(12):1-4+16.
CHEN J, JIANG H F, MA M S, et al. Preparation of flower-like mesoporous silica spheres and their drug sustained release properties[J]. Technology & Development of Chemical Industry, 2019, 48(12): 1-4+16(in Chinese).
[29] 韩玉,瞿成良,王亚斌,等.超小孔径树枝状介孔二氧化硅纳米球的制备和表征[J].人工晶体学报,2020,49(4):684-688+704.
HAN Y, QU C L, WANG Y B, et al. Synthesis and characterization of dendritic mesoporous silica nanospheres (DMSNs) with extremely tiny pore size[J]. Journal of Synthetic Crystals, 2020, 49(4): 684-688+704(in Chinese).
[30] 刘群, 张志华, 刘源, 等. 二氧化硅气凝胶对挥发性有机污染物的吸附性能研究[J]. 化学通报, 2020, 83(6): 552-556+507.
LIU Q, ZHANG Z H, LIU Y, et al. Adsorption properties of silica aerogels for volatile organic pollutants[J]. Bulletin of chemistry, 2020, 83 (6): 552-556+507(in Chinese).
[31] BISWAS N. Modified mesoporous silica nanoparticles for enhancing oral bioavailability and antihypertensive activity of poorly water soluble valsartan[J]. European Journal of Pharmaceutical Sciences, 2017, 99: 152-160.
[32] 周韵秋,冀素平,刘聪,等.白藜芦醇聚合物胶束的制备及质量评价[J].中国医药生物技术,2020,15(1):25-31.
ZHOU Y Q, JI S P, LIU C, et al. Preparation and quality evaluation of resveratrol loaded polymeric micelles[J]. Chinese Medicinal Biotechnology, 2020, 15(1): 25-31(in Chinese).
[33] 吕江维,魏亚青,王鹏光,等.茴拉西坦/介孔二氧化硅的制备及释药性能[J].人工晶体学报,2019,48(9):1718-1725+1753.
LYU J W, WEI Y Q, WANG P G, et al. Preparation and drug release performance of aniracetam/mesoporous silica[J]. Journal of Synthetic Crystals, 2019, 48(9): 1718-1725+1753(in Chinese).
[34] 国家药典委员会. 中华人民共和国药典, 四部[S]. 北京: 中国医药科技出版社, 2020: 132.
National Pharmacopoeia Committee. Pharmacopoeia of the People’s Republic of China, volume 4[S]. Beijing: China Pharmaceutical Science and Technology Press, 2020: 132(in Chinese).
[35] 邓姣,刘鑫,郑敏,等.静电纺丝制备白藜芦醇固体分散体及其性能[J/OL].食品科学:1-11[2021-02-06]. http://kns.cnki.net/kcms/detail/11.2206.TS.20201229.0917.016.html.
DENG J, LIU X, ZHENG M, et al. Preparation and properties of resveratrol solid dispersion by electrospinning [J/OL]. Food Science: 1-11[2021-02-06] http://kns.cnki.net/kcms/detail/11.2206.TS.20201229.0917.016.html(in Chinese).