欢迎访问《人工晶体学报》官方网站,今天是 分享到:

人工晶体学报 ›› 2021, Vol. 50 ›› Issue (2): 375-380.

• 研究论文 • 上一篇    下一篇

大豆脲酶诱导碳酸钙沉淀的多因素影响分析

原华1,2, 刘康2, 原耀楠3, 冯佳星4   

  1. 1.河南大学,岩土与轨道交通工程研究所,开封 475004;
    2.河南大学土木建筑学院,开封 475004;
    3.河南建业置地焦作有限公司,焦作 454150;
    4.北京中科宏泰顾问集团有限公司,北京 101100
  • 收稿日期:2021-01-04 发布日期:2021-03-24
  • 作者简介:原 华(1982—),女,河南省人,博士,副教授。E-mail:yuanhuazzl@163.com
  • 基金资助:
    河南省重点研发与推广专项(科技攻关)(202102310247)

Affecting Analysis of Multi-Factors on Soybean Urease-Induced Calcium Carbonate Precipitation

YUAN Hua1,2, LIU Kang2, YUAN Yaonan3, FENG Jiaxing4   

  1. 1.Institute of Geotechnical and Rail Transport Engineering, Henan University, Kaifeng 475004, China;
    2.School of Civil Engineering and Architecture, Henan University, Kaifeng 475004, China;
    3.Henan Jianye Land Jiaozuo Co., Ltd., Jiaozuo 454150, China;
    4.Beijing Zhongke Hongtai Consulting Group Co., Ltd., Beijing 101100, China
  • Received:2021-01-04 Published:2021-03-24

摘要: 本文探究了多个影响因素对大豆脲酶诱导碳酸钙沉淀(SICP)的影响,以优选出主要影响因素并提供其最佳范围。首先分析了脲酶浓度和温度对脲酶活性的影响;之后通过正交实验设计,进行25种工况的SICP水溶液实验,研究不同因素组合下Ca2+利用率的变化规律;最后借助扫描电子显微镜观测不同工况下生成碳酸钙的形态。结果表明:低温有利于脲酶的保存及活性发挥,5 ℃时脲酶活性能保持21 d以上;同一温度下,脲酶浓度越大,脲酶初始活性越高,脲酶完全失活所需时间越短。pH值、脲酶与胶结液体积比是影响Ca2+利用率的主要因素。为达到较高的Ca2+利用率,脲酶和胶结液最佳体积比为1,氯化钙与尿素最佳浓度比为1.5,Ca2+最佳浓度为1 mol/L。当脲酶浓度较低时生成的六面体状碳酸钙较多;随着脲酶浓度的增大,所沉淀的碳酸钙向球形转变。大豆中富含的天冬氨酸是控制碳酸钙形态的重要因素。

关键词: 大豆脲酶诱导碳酸钙沉淀, Ca2+利用率, 球形碳酸钙, 天冬氨酸, 多因素, 正交实验

Abstract: The effects of multiple influencing factors on soybean urease-induced calcium carbonate precipitation (SICP) were explored to select the main influencing factors and provided their optimal ranges. Firstly, the influence of urease concentration and temperature on urease activity were analyzed. After that, based on orthogonal test design, 25 test conditions of SICP aqueous solution tests were conducted, and the variation law of Ca2+ utilization ratio was studied through the combination of different factors. Also the morphology of CaCO3 was observed with SEM in diverse test conditions. The results show that low temperature delivers benefits to urease preservation and its activity exertion, urease activity can be preserved over 21 d with 5 ℃. At the same temperature, the greater the urease concentration is, the higher the initial activity of urease boasts and the shorter the time required for complete inactivation of urease. The pH value and the volume ratio of urease to cementation solution are the main factors affecting the Ca2+ utilization ratio. In order to achieve a higher Ca2+ utilization ratio, the optimum volume ratio of urease to cementation solution could be 1 while the optimal concentration ratio of CaCl2 to urea is 1.5, and the best Ca2+ concentration is 1 mol/L. More hexahedral CaCO3 is produced, when the urease concentration is low, and the shape of precipitated CaCO3 turns into spherical as the urease concentration increases. Aspartic acid which is rich in soybeans, is an significant factor in controlling the form of CaCO3.

Key words: soybean urease-induced calcium carbonate precipitation, Ca2+ utilization ratio, spherical CaCO3, aspartic acid, multi-factors, orthogonal test

中图分类号: