Synthesis, HSA Binding, and Cytotoxic Activity of Copper(Ⅱ) Complex Constructed by P-Chlorobenzoic Acid

Abstract

Abstract: In this paper, a copper(Ⅱ) complex [Cu(pcba)₂·(phen)(H₂O)] (pcba=p-chlorbenzoic acid, phen=1,10-phenanthroline) was synthesized. The structure of the complex belongs to triclinic, P1 space group, with a=0.790 98(2) nm,b=1.072 40(4) nm, c=1.487 19(6) nm, α=100.613(3)°, β=95.239(3)°, γ=108.334(3)°, Z=2, D_c=1.638 g·cm^-3, F(000)=582 unit cell parameters. The final R₁=0.035 9, wR₂=0.089 1. The interaction between HSA (human serum albumin) and the complex was evaluated by UV-Vis and fluorescence spectroscopy. The complex quenched the intrinsic fluorescence of HSA via static quenching with K_sv=2.35×10⁵ L·mol^-1, the quenching rate constant K_q=2.35×10¹³ L·mol^-1·s^-1, the binding constant K_a=2.14×10¹³ L·mol^-1, the binding site n=2.37. Furthermore, the proliferation inhibition effect of the complex on the cells A549, Hela and HepG2 was studied.

Key words: complex, copper complex, human serum albumi, P-chlorobenzoic acid, copper(Ⅱ), HSA binding, cytotoxic activity

CLC Number:

ZENG Zhenfang, YUAN Fang, HUANG Qiuping, PANG Huayu, YANG Honglan, HUANG Qiuchan. Synthesis, HSA Binding, and Cytotoxic Activity of Copper(Ⅱ) Complex Constructed by P-Chlorobenzoic Acid[J]. JOURNAL OF SYNTHETIC CRYSTALS, 2022, 51(1): 126-131.

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