氨基酸/氧化石墨烯/羟基磷灰石复合材料在酸蚀人牙釉质体外再矿化中的应用

摘要/Abstract

摘要： 本文研究了甘氨酸(Gly)和氧化石墨烯(GO)复合改性羟基磷灰石(Gly/GO/HA),以及天冬氨酸(Asp)和GO复合改性羟基磷灰石(Asp/GO/HA)对酸蚀人牙釉质(HE)体外再矿化的影响。通过CCK-8法测定不同复合材料的细胞毒性,通过场发射扫描电镜、EDX能谱分析、X射线衍射和显微硬度测试,对再矿化前/后HE表面及剖面形貌、表面元素组成、表面成分结构和表面力学性能进行表征。结果表明:Gly/GO/HA和Asp/GO/HA均有良好的安全性和生物相容性,且均能使酸蚀HE得到一定的修复,其中Gly/GO/HA对酸蚀HE表面和深层脱矿的修复效果优于Asp/GO/HA。

关键词: 氨基酸, 氧化石墨烯, 羟基磷灰石, 牙釉质, 再矿化, 细胞毒性

Abstract: Effects of glycine (Gly) and graphene oxide (GO) modified hydroxyapatite (Gly/GO/HA), and aspartic acid (Asp) and GO modified hydroxyapatite (Asp/GO/HA) on in vitro remineralization of acid-etched human enamel (HE) were investigated. The cytotoxicity of these composites was studied by CCK-8 method, the surface and cross-section morphology, surface element composition, surface composition structure and surface mechanical properties of HE before and after remineralization were characterized by field emission scanning electron microscope, EDX energy spectrum analysis, X-ray diffraction and microhardness test. The results show that Gly/GO/HA and Asp/GO/HA both have good safety and biocompatibility, and both can repair the acid-etched HE to a certain extent, among which Gly/GO/HA is better than Asp/GO/HA in repairing the surface and deep demineralization of the acid-etched HE.

Key words: amino acid, graphene oxide, hydroxyapatite, enamel, remineralization, cytotoxicity

中图分类号:

蔡英, 钏定泽, 陈庆华, 刘继涛. 氨基酸/氧化石墨烯/羟基磷灰石复合材料在酸蚀人牙釉质体外再矿化中的应用[J]. 人工晶体学报, 2022, 51(3): 516-522.

CAI Ying, CHUAN Dingze, CHEN Qinghua, LIU Jitao. Amino Acids/Graphene Oxide/Hydroxyapatite Composites on in vitro Remineralization of Acid-Etched Human Enamel[J]. JOURNAL OF SYNTHETIC CRYSTALS, 2022, 51(3): 516-522.

参考文献

[1] DING L J, HAN S L, WANG K, et al. Remineralization of enamel caries by an amelogenin-derived peptide and fluoride in vitro[J]. Regenerative Biomaterials, 2020, 7(3): 283-292.
[2] RUAN Q C, MORADIAN-OLDAK J. Amelogenin and enamel biomimetics[J]. Journal of Materials Chemistry B, 2015, 3(16): 3112-3129.
[3] HE L H, SWAIN M V. Understanding the mechanical behaviour of human enamel from its structural and compositional characteristics[J]. Journal of the Mechanical Behavior of Biomedical Materials, 2008, 1(1): 18-29.
[4] HAMBA H, NAKAMURA K, NIKAIDO T, et al. Remineralization of enamel subsurface lesions using toothpaste containing tricalcium phosphate and fluoride: an in vitro μCT analysis[J]. BMC Oral Health, 2020, 20(1): 292.
[5] 程亚楠,吴毓聪,毛秋华,等.渗透树脂联合生物活性玻璃修复脱矿牙釉质的效果及稳定性[J].中国组织工程研究,2021,25(22):3522-3526.
CHENG Y N, WU Y C, MAO Q H, et al. Restoration effect and stability of resin infiltration combined with bioactive glass on demineralized tooth enamel[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3522-3526(in Chinese).
[6] 俞晓峰,卢友光,陈舟,等.贝哥仕促进釉质再矿化的体外研究[J].福建医科大学学报,2020,54(5):348-351.
YU X F, LU Y G, CHEN Z, et al. Effect of bgfrsh on the remineralization of early enamel caries in vitro[J]. Journal of Fujian Medical University, 2020, 54(5): 348-351(in Chinese).
[7] BONCHEV A, VASILEVA R, DYULGEROVA E, et al. Self-assembling peptide P₁₁-4: a biomimetic agent for enamel remineralization[J]. International Journal of Peptide Research and Therapeutics, 2021, 27(2): 899-907.
[8] 赵华蕾,楚金普.牙釉质仿生再矿化的研究进展[J].实用口腔医学杂志,2019,35(2):298-302.
ZHAO H L, CHU J P. Research progress of biomimetic remineralization of enamel[J]. Journal of Practical Stomatology, 2019, 35(2): 298-302(in Chinese).
[9] 金启予,邓淑丽,胡济安.仿生生物分子材料在牙体硬组织再矿化中的应用[J].口腔医学,2020,40(11):1037-1040.
JIN Q Y, DENG S L, HU J A. Application of biomimetic biomolecular materials in remineralization of hard tissue of dental body[J]. Stomatology, 2020, 40(11): 1037-1040(in Chinese).
[10] DING L J, HAN S L, PENG X, et al. Tuftelin-derived peptide facilitates remineralization of initial enamel caries in vitro[J]. Journal of Biomedical Materials Research Part B, Applied Biomaterials, 2020, 108(8): 3261-3269.
[11] MOHAMED R N, BASHA S, AL-THOMALI Y, et al. Self-assembling peptide P₁₁-4 in remineralization of enamel caries - a systematic review of in-vitro studies[J]. Acta Odontologica Scandinavica, 2021, 79(2): 139-146.
[12] KARUMURI S, MANDAVA J, PAMIDIMUKKALA S, et al. Efficacy of hydroxyapatite and silica nanoparticles on erosive lesions remineralization[J]. Journal of Conservative Dentistry: JCD, 2020, 23(3): 265-269.
[13] 刘继涛, 陈庆华.牙釉质的结构和力学性能研究进展[J]. 口腔医学研究, 2020, 36(3):3.
LIU J T, CHEN Q H. Research Progress on the structure and mechanical properties of enamel[J]. Stomatological Research, 2020, 36(3): 3(in Chinese).
[14] GLIMCHER M J, LEVINE P T. Studies of the proteins, peptides and free amino acids of mature bovine enamel[J]. The Biochemical Journal, 1966, 98(3): 742-753.
[15] van der LINDEN A H I M, BOOIJ M, TEN BOSCH J J, et al. Protein loss of bovine dental enamel during in-vitro subsurface demineralization[J]. Archives of Oral Biology, 1985, 30(9): 645-650.
[16] DUVERGER O, BENIASH E, MORASSO M I. Keratins as components of the enamel organic matrix[J]. Matrix Biology, 2016, 52/53/54: 260-265.
[17] LI L, MAO C Y, WANG J M, et al. Bio-inspired enamel repair via Glu-directed assembly of apatite nanoparticles: an approach to biomaterials with optimal characteristics[J]. Advanced Materials (Deerfield Beach, Fla), 2011, 23(40): 4695-4701.
[18] WU X G, ZHAO X, LI Y, et al. In situ synthesis carbonated hydroxyapatite layers on enamel slices with acidic amino acids by a novel two-step method[J]. Materials Science and Engineering: C, 2015, 54: 150-157.
[19] 刘继涛, 钏定泽, 杨泽斌,等. 氨基酸/羟基磷灰石复合材料的制备与表征及其在酸蚀牛牙釉质体外再矿化中的应用[J]. 材料工程, 2020, 48(2):100-107.
LIU J T, CHUAN D Z, YANG Z B, et al. Preparation and characterization of amino acid/hydroxyapatite composites and their application in in vitro remineralization of acid eroded bovine enamel[J]. Materials engineering, 2020, 48 (2): 100-107(in Chinese).
[20] 龚秀文,薛秀玲,陈小奕,等.氨基化氧化石墨烯(AGO)的制备及其对六价铬的吸附机制[J].环境化学,2021,40(3):851-858.
GONG X W, XUE X L, CHEN X Y, et al. Synthesis of amino-functionalized graphene oxide and adsorption performance to remove Cr(Ⅵ) in water[J]. Environmental Chemistry, 2021, 40(3): 851-858(in Chinese).
[21] KIM F, COTE L J, HUANG J X. Graphene oxide: surface activity and two-dimensional assembly[J]. Advanced Materials (Deerfield Beach, Fla), 2010, 22(17): 1954-1958.
[22] DONG C C, LU J, QIU B C, et al. Developing stretchable and graphene-oxide-based hydrogel for the removal of organic pollutants and metal ions[J]. Applied Catalysis B: Environmental, 2018, 222: 146-156.
[23] 刘继涛,钏定泽,陈希亮,等.氧化石墨烯/羟基磷灰石复合材料对酸蚀牛牙釉质体外再矿化的影响[J].人工晶体学报,2020,49(1):131-137.
LIU J T, CHUAN D Z, CHEN X L, et al. Effect of graphene oxide/hydroxyapatite composite on in vitro remineralization of acid-etched bovine enamel[J]. Journal of Synthetic Crystals, 2020, 49(1): 131-137(in Chinese).